Agrn^tm3Jrs
Targeted Allele Detail

Summary

• Symbol: Agrn^tm3Jrs
• Name: agrin; targeted mutation 3, Joshua R Sanes
• MGI ID: MGI:1930979
• Synonyms: agrn^deltaZ, AGZ^-
• Gene: Agrn Location: Chr4:156249747-156281945 bp, - strand Genetic Position: Chr4, 88.55 cM
• Alliance: Agrn^tm3Jrs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55407
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Deletion of exons 32 and 33, encoding the "z" alternative splice exons as follows: A lox-P flanked neomycin cassette replaced exons 32 and 33, and was excised in ES cells prior to blastocyst injection via Cre-mediated recombination. The final allele has a single loxP site in place of the two exons. (J:55407)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agrn Mutation: 102 strains or lines available

References

• Original: J:55407 Burgess RW, et al., Alternatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction. Neuron. 1999 May;23(1):33-44
• All: 5 reference(s)