Nr3c1^tm3Gsc
Targeted Allele Detail

Summary

• Symbol: Nr3c1^tm3Gsc
• Name: nuclear receptor subfamily 3, group C, member 1; targeted mutation 3, Gunther Schutz
• MGI ID: MGI:1931329
• Synonyms: GR^D, GR^dim
• Gene: Nr3c1 Location: Chr18:39543598-39652474 bp, - strand Genetic Position: Chr18, 21.09 cM
• Alliance: Nr3c1^tm3Gsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67720
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: A point mutation was introduced into exon 4 to change alanine codon 474 to threonine (p.A474T). The loxP site flanked neomycin resistance gene cassette that was inserted into intron 3 was removed via Cre-mediated recombination in ES cells prior to blastocyst injection. This mutation causes a defect in GRE-mediated transactivation and prevents dimerization. (J:67720, J:121199)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nr3c1 Mutation: 35 strains or lines available

References

• Original: J:67720 Reichardt HM, et al., DNA binding of the glucocorticoid receptor is not essential for survival. Cell. 1998 May 15;93(4):531-41
• All: 42 reference(s)