Pou2af1^tm1Rgr
Targeted Allele Detail

Summary

• Symbol: Pou2af1^tm1Rgr
• Name: POU domain, class 2, associating factor 1; targeted mutation 1, Robert Roeder
• MGI ID: MGI:1931863
• Synonyms: OCA-B-
• Gene: Pou2af1 Location: Chr9:51125008-51151380 bp, + strand Genetic Position: Chr9, 27.95 cM
• Alliance: Pou2af1^tm1Rgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35785
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced exons 2, 3, and 4. Northern blot analysis on spleen RNA from homozygous mice confirmed that no mRNA was expressed from this allele and immunoblot analysis on bone marrow derived cell lines from homozygous mice demonstrated an absence of encoded protein. (J:35785)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pou2af1 Mutation: 15 strains or lines available

Notes

ES cell line = E14 (129P2/OlaHsd) or CJ7 (129S1/Sv-p⁺ Tyr⁺ Kitl⁺).

References

• Original: J:35785 Kim U, et al., The B-cell-specific transcription coactivator OCA-B/OBF-1/Bob-1 is essential for normal production of immunoglobulin isotypes. Nature. 1996 Oct 10;383(6600):542-7
• All: 14 reference(s)