Kmt2a^tm1Sjk
Targeted Allele Detail

Summary

• Symbol: Kmt2a^tm1Sjk
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1, Stanley J Korsmeyer
• MGI ID: MGI:1932081
• Synonyms: MLL-
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^tm1Sjk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29958
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ-neomycin resistance cassette was inserted in-frame into exon 3b and placed lacZ under control of the endogenous promoter. RT-PCR studies of homozygous E10.5 embryos showed that trancripts lacking the sequences encoding the functional domains were produced from this allele. (J:29958)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 135 strains or lines available

Notes

A posterior shift in the anterior boundaries of expression of Hoxa7 and Hoxc9 occurs in Mll^tm1Sjk heterozygotes, while expression of these genes is completely abolished in homozygous nulls (J:29958).

References

• Original: J:29958 Yu BD, et al., Altered Hox expression and segmental identity in Mll-mutant mice. Nature. 1995 Nov 30;378(6556):505-8
• All: 12 reference(s)