Spp1^tm1Blh
Targeted Allele Detail

Summary

• Symbol: Spp1^tm1Blh
• Name: secreted phosphoprotein 1; targeted mutation 1, Brigid L Hogan
• MGI ID: MGI:1932083
• Synonyms: eta1, Eta-1, Opn-, OPN^-, OPN-KO
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^tm1Blh page

Reduced diaphragm and cardiac fibrosis in Dmd^mdx/Y Spp1^tm1Blh/Spp1^tm1Blh and Dmd^mdx/Dmd^mdx Spp1^tm1Blh/Spp1^tm1Blh mice compared to Dmd^mdx/Dmd^mdx mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46863
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK neomycin resistance cassette replaced exons 4-7 of the Spp1 gene. No Spp1 transcript was detected by RT-PCR in embryonic fibroblasts or adult kidney from homozygous mutant animals. Spp1 transcript was not detected in homozygous mutant animals by in situ hybridization. Immunohistochemistry studies of adult kidney and bone did not detect protein in homozygous mutant mice. (J:46863)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 43 strains or lines available

References

• Original: J:46863 Liaw L, et al., Altered wound healing in mice lacking a functional osteopontin gene (spp1). J Clin Invest. 1998 Apr 1;101(7):1468-78
• All: 159 reference(s)