Spp1^tm1Rit
Targeted Allele Detail

Summary

• Symbol: Spp1^tm1Rit
• Name: secreted phosphoprotein 1; targeted mutation 1, Susan R Rittling
• MGI ID: MGI:1932084
• Synonyms: Eta-1^-, Opn-
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^tm1Rit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56535
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a neomycin resistance cassette into exon 6 immediately 5' of the RGD sequence disrupted the Spp1 gene. Northern analysis of homozygous mutant kidney detected low levels of a high molecular weight RNA species, but no wild-type or truncated Spp1 transcript. Immunoblot analysis detected low levels of a truncated form of the protein in bone but not in other tissues, fluids, or cells of homozygous mutant mice. (J:56535)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 43 strains or lines available

References

• Original: J:56535 Rittling SR, et al., Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. J Bone Miner Res. 1998 Jul;13(7):1101-11
• All: 95 reference(s)