Zp3^tm1Pmw
Targeted Allele Detail

Summary

• Symbol: Zp3^tm1Pmw
• Name: zona pellucida glycoprotein 3; targeted mutation 1, Paul W Wasserman
• MGI ID: MGI:1932681
• Synonyms: mZP3^-
• Gene: Zp3 Location: Chr5:136008959-136017478 bp, + strand Genetic Position: Chr5, 75.62 cM
• Alliance: Zp3^tm1Pmw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33254
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2, intron 2 and exon 3 were replaced by a pGK-neomycin cassette. Homozygous mutant female mice were analyzed by Northern and Western blotting to establish that the mRNA and protein were not present in growing oocytes. (J:33254)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zp3 Mutation: 40 strains or lines available

References

• Original: J:33254 Liu C, et al., Targeted disruption of the mZP3 gene results in production of eggs lacking a zona pellucida and infertility in female mice. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5431-6
• All: 5 reference(s)