Tob1^tm1Tya
Targeted Allele Detail

Summary

• Symbol: Tob1^tm1Tya
• Name: transducer of ErbB-2.1; targeted mutation 1, Tadashi Yamamoto
• MGI ID: MGI:1933294
• Synonyms: tob-
• Gene: Tob1 Location: Chr11:94102280-94106321 bp, + strand Genetic Position: Chr11, 58.9 cM, cytoband C
• Alliance: Tob1^tm1Tya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66504
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neomycin resistance cassette was inserted into the only exon of the gene. Western blot analysis of primary embryonic fibroblasts detected only one of the two wild-type forms of the protein in homozygous mutant mice. Northern blot analysis did not detect transcript in osteoblast lineage cells derived from calvariae of homozygous mutant newborns. Also, BMP2-stimulated DNA synthesis was enhanced in these cells compared to wild-type. (J:66504)

Expression

In Mice Carrying this Mutation:	2 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tob1^tm1Tya

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Tob1 Mutation: 30 strains or lines available

References

• Original: J:66504 Yoshida Y, et al., Negative regulation of BMP/Smad signaling by Tob in osteoblasts. Cell. 2000 Dec 22;103(7):1085-97
• All: 13 reference(s)