Shc2^tm1Paw
Targeted Allele Detail

Summary

• Symbol: Shc2^tm1Paw
• Name: SHC (Src homology 2 domain containing) transforming protein 2; targeted mutation 1, Tony Pawson
• MGI ID: MGI:1933344
• Synonyms: ShcB-
• Gene: Shc2 Location: Chr10:79453885-79473752 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Shc2^tm1Paw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66508
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Insertion of an IRES-LacZ sequence followed by a PGK-neomycin resistance cassette into exon 5, which encodes the phosphotyrosine binding (PTB) domain. The authors predict that this insertion would cause the mutant allele to terminate translation within the PTB domain. Western blot analysis and immunoprecipitation studies did not detect wild-type protein in 4 week old homozygous mutant brain. (J:66508)

Expression

In Mice Carrying this Mutation:	28 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Shc2 Mutation: 33 strains or lines available

References

• Original: J:66508 Sakai R, et al., The mammalian ShcB and ShcC phosphotyrosine docking proteins function in the maturation of sensory and sympathetic neurons. Neuron. 2000 Dec;28(3):819-33
• All: 2 reference(s)