Pou5f1^tm1Cgre
Targeted Allele Detail

Summary

• Symbol: Pou5f1^tm1Cgre
• Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Centre for Genome Research
• MGI ID: MGI:1933381
• Synonyms: Oct4-
• Gene: Pou5f1 Location: Chr17:35816929-35821674 bp, + strand Genetic Position: Chr17, 18.69 cM
• Alliance: Pou5f1^tm1Cgre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51139
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An internal ribosome entry site-lacZ-neomycin resistance cassette (IRES-beta-geo) replaced sequences in exons 2-5 encoding the two DNA binding domains and the carboxy terminal transactivation site. Immunostaining did not detect wild-type protein in 3 of 15 2.5 day morulae from an intercross of heterozygous mutant animals. (J:51139)

Expression

In Mice Carrying this Mutation:	3 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou5f1 Mutation: 82 strains or lines available

References

• Original: J:51139 Nichols J, et al., Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell. 1998 Oct 30;95(3):379-91
• All: 5 reference(s)