Sp5^tm1Rbe
Targeted Allele Detail

Summary

• Symbol: Sp5^tm1Rbe
• Name: trans-acting transcription factor 5; targeted mutation 1, Rosa Beddington
• MGI ID: MGI:1933409
• Synonyms: Sp5^lacZ
• Gene: Sp5 Location: Chr2:70305267-70308070 bp, + strand Genetic Position: Chr2, 41.49 cM
• Alliance: Sp5^tm1Rbe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65743
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-LacZ-floxed-MC1-neomycin resistance cassette replaced much of the open reading frame. The authors predict that the targeted allele would encode a bicistronic message under control of the endogenous promoter that expresses both a truncated N-terminal SP5 protein and beta-galactosidase. (J:65743)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sp5 Mutation: 15 strains or lines available

References

• Original: J:65743 Harrison SM, et al., Sp5, a new member of the Sp1 family, is dynamically expressed during development and genetically interacts with brachyury. Dev Biol. 2000 Nov 15;227(2):358-72
• All: 6 reference(s)