Cbfb^tm1Tno
Targeted Allele Detail

Summary

• Symbol: Cbfb^tm1Tno
• Name: core binding factor beta; targeted mutation 1, Tetsuo Noda
• MGI ID: MGI:1933624
• Synonyms: PEBP2beta/CBFbeta (-), Pebpb2^-
• Gene: Cbfb Location: Chr8:105897306-105944621 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cbfb^tm1Tno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40963
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exon 1, which contains the translation start site and the sequences encoding the first 26 amino acids of the protein. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that no detectable transcript is produced from this allele, and western blot analysis on embryo extracts derived from homozygous mice confirmed that no stable protein is made. (J:40963)

Expression

In Mice Carrying this Mutation:	58 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cbfb Mutation: 36 strains or lines available

References

• Original: J:40963 Niki M, et al., Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor, polyomavirus enhancer binding protein 2/core binding factor. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5697-702
• All: 4 reference(s)