Cd44^tm1Hbg
Targeted Allele Detail

Summary

• Symbol: Cd44^tm1Hbg
• Name: CD44 antigen; targeted mutation 1, Frank Hilberg
• MGI ID: MGI:1934008
• Synonyms: CD44-
• Gene: Cd44 Location: Chr2:102641486-102732010 bp, - strand Genetic Position: Chr2, 54.13 cM
• Alliance: Cd44^tm1Hbg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68069
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A promoterless lacZ gene followed by a neomycin selection cassette was fused in frame directly following the translation start codon. Northern blot analysis on RNA derived from E13.5 homozygous embryos demonstrated that no wild-type transcript was detectable. Flow cytometry analysis on cells derived from thymus, spleen and lymph nodes of homozygous mice confirmed that no stable encoded protein was expressed on the cell surface. (J:68069)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Cd44 Mutation: 72 strains or lines available

References

• Original: J:68069 Protin U, et al., CD44-deficient mice develop normally with changes in subpopulations and recirculation of lymphocyte subsets. J Immunol. 1999 Nov 1;163(9):4917-23
• All: 95 reference(s)