Hfe^tm1Gfn
Targeted Allele Detail

Summary

• Symbol: Hfe^tm1Gfn
• Name: homeostatic iron regulator; targeted mutation 1, Susan Gilfillan
• MGI ID: MGI:1934013
• Synonyms: Hfe⁰
• Gene: Hfe Location: Chr13:23886017-23894837 bp, - strand Genetic Position: Chr13, 9.88 cM, cytoband A2-A4
• Alliance: Hfe^tm1Gfn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68025
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: 2.5 kb of sequence containing the second and third exons, which encode the alpha1 and alpha2 putative ligand binding domains, were replaced with a floxed PGK neomycin selection cassette. Northern blots prepared with total RNA isolated from kidney and liver tissue did not detect full length mRNA in homozygous mutant mice. (J:68025)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hfe Mutation: 35 strains or lines available

References

• Original: J:68025 Bahram S, et al., Experimental hemochromatosis due to MHC class I HFE deficiency: immune status and iron metabolism. Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13312-7
• All: 29 reference(s)