Ighm^tm1Che
Targeted Allele Detail

Summary

• Symbol: Ighm^tm1Che
• Name: immunoglobulin heavy constant mu; targeted mutation 1, Jianzhu Chen
• MGI ID: MGI:1934014
• Synonyms: muS-, secreted IgM^-, sIgM^-, Smu^-
• Gene: Ighm Location: Chr12:113382446-113386350 bp, - strand Genetic Position: Chr12, 62.1 cM, cytoband F1-2
• Alliance: Ighm^tm1Che page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47425
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was constructed in which the exon encoding the secreted form of the immunoglobulin mu (IgM) heavy chain and its three downstream poly(A) sites were replaced by a cDNA fragment encoding the constant region mu4 exon and the exon encoding the membrane-bound form of IgM. The resultant homozygous mutant mice had B cells that did not secrete IgM, but did express membrane-bound IgM. (J:47425)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ighm Mutation: 56 strains or lines available

References

• Original: J:47425 Boes M, et al., Enhanced B-1 cell development, but impaired IgG antibody responses in mice deficient in secreted IgM. J Immunol. 1998 May 15;160(10):4776-87
• All: 57 reference(s)