Il4^tm1Gru
Targeted Allele Detail

Summary

• Symbol: Il4^tm1Gru
• Name: interleukin 4; targeted mutation 1, Michael Grusby
• MGI ID: MGI:1934015
• Synonyms: mIL-4
• Gene: Il4 Location: Chr11:53503287-53509492 bp, - strand Genetic Position: Chr11, 31.97 cM
• Alliance: Il4^tm1Gru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53107
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A modified version of exon 4 of the gene was inserted between the last codon and the translational stop site of the gene. This modified version of exon 4 contained a transmembrane anchor. In addition, a neomycin resistance cassette was inserted 150 nucleotides downstream of the polyadenylation signal of the gene. These modifications allowed production of mice that express a membrane-bound form of the protein. (J:53107)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il4 Mutation: 46 strains or lines available

Notes

This mutation resulted in mice that express Il4 in a membrane-bound form for use in identifying and characterizing Il4-producing cells.

References

• Original: J:53107 Ho IC, et al., Marking IL-4-producing cells by knock-in of the IL-4 gene. Int Immunol. 1999 Feb;11(2):243-7
• All: 1 reference(s)