Sell^tm1Hyn
Targeted Allele Detail

Summary

• Symbol: Sell^tm1Hyn
• Name: selectin, lymphocyte; targeted mutation 1, Richard Hynes
• MGI ID: MGI:1934022
• Synonyms: L^-, L-Sel^-
• Gene: Sell Location: Chr1:163889556-163908354 bp, + strand Genetic Position: Chr1, 71.37 cM
• Alliance: Sell^tm1Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57973
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. In order to create a triple knockout, ES cells heterozygote for the Selp^tm1Hyn and Sele^tm1Hyn null alleles in cis to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of all three encoded proteins in leukocytes isolated from homozygotes. This targeted mutation occurred in cis with the other two null mutations. Due to their genomic proximity, the three null alleles should propagate together through the offspring. (J:57973)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Sell Mutation: 42 strains or lines available

References

• Original: J:57973 Robinson SD, et al., Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11452-7
• All: 24 reference(s)