Soat1^tm1Ishi
Targeted Allele Detail

Summary

• Symbol: Soat1^tm1Ishi
• Name: sterol O-acyltransferase 1; targeted mutation 1, Shun Ishibashi
• MGI ID: MGI:1934032
• Synonyms: ACAT-1
• Gene: Soat1 Location: Chr1:156255678-156301898 bp, - strand Genetic Position: Chr1, 67.71 cM
• Alliance: Soat1^tm1Ishi page

Narrow eye fissures and atrophic meibomian glands in Soat1^tm1Ishi/Soat1^tm1Ishi mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63468
• Parent Cell Line: JH1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 4 kb of sequence encoding the first transmembrane domain and a conserved serine. Northern blot analyses of homozygous mutant mice did not detect any transcripts in macrophages, adrenal glands, ovaries and testes. Western blot analyses of macrophages, adrenal glands, ovaries, and testes did not detect the protein in homozygous mutant mice. (J:63468)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Soat1 Mutation: 42 strains or lines available

References

• Original: J:63468 Yagyu H, et al., Absence of ACAT-1 attenuates atherosclerosis but causes dry eye and cutaneous xanthomatosis in mice with congenital hyperlipidemia. J Biol Chem. 2000 Jul 14;275(28):21324-30
• All: 2 reference(s)