Flt1^tm1Msh
Targeted Allele Detail

Summary

• Symbol: Flt1^tm1Msh
• Name: FMS-like tyrosine kinase 1; targeted mutation 1, Masabumi Shibuya
• MGI ID: MGI:1934260
• Synonyms: Flt-1^TK-, flt tk^-, VEGFR1-DeltaTKD
• Gene: Flt1 Location: Chr5:147498414-147662821 bp, - strand Genetic Position: Chr5, 87.01 cM, cytoband G
• Alliance: Flt1^tm1Msh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49507
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced exon 17, which encodes the amino terminal region of the tyrosine kinase domain. Northern blot analysis of homozygous mutant embryos using a 5' cDNA probe detected a ~3kb mRNA, and detected no 7kb full length transcript using a 3' probe. Immunoblot analysis of lung tissue from homozygous mutant mice detected the endogenous soluble form of the protein, and a novel truncated form that lacks the cytoplasmic region. No full length protein was expressed from this allele. (J:49507)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Flt1 Mutation: 76 strains or lines available

References

• Original: J:49507 Hiratsuka S, et al., Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9349-54
• All: 38 reference(s)