Hspg2^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Hspg2^tm1Ref
• Name: perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:1934892
• Gene: Hspg2 Location: Chr4:137196080-137297941 bp, + strand Genetic Position: Chr4, 69.93 cM
• Alliance: Hspg2^tm1Ref page

Hemopericardium development in E10.5 Hspg2^tm1Ref/Hspg2^tm1Ref embryos

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58700
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A neomycin/thymidine kinase cassette flanked by single loxP sites was inserted into intron 5 and a single loxP site was inserted into intron 6 of the gene. Correctly targeted ES cells were treated with cre recombinase and, in resultant clones, the sequence between the outermost loxP sites (including the neomycin/thymidine kinase cassette, part of intron 5, exon 6, and part of intron 6) was deleted. (J:58700)

Disease models

Expression

In Structures Affected by this Mutation:

32 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hspg2 Mutation: 310 strains or lines available

References

• Original: J:58700 Costell M, et al., Perlecan maintains the integrity of cartilage and some basement membranes. J Cell Biol. 1999 Nov 29;147(5):1109-22
• All: 10 reference(s)