Dag1^tm1Kcam
Targeted Allele Detail

Summary

• Symbol: Dag1^tm1Kcam
• Name: dystroglycan 1; targeted mutation 1, Kevin P Campbell
• MGI ID: MGI:1934902
• Synonyms: Dag1^neo2, DG-
• Gene: Dag1 Location: Chr9:108082060-108141176 bp, - strand Genetic Position: Chr9, 59.08 cM
• Alliance: Dag1^tm1Kcam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40601
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced part of exon 2, including the splice acceptor site and a large part of the coding sequence. No detectable transcript was produced from this allele in homozygous embryos (data not shown) and immunohistochemistry experiments on homozygous embryos confirmed that no detectable encoded protein was present. (J:40601)

Disease models

Expression

In Mice Carrying this Mutation:	22 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dag1 Mutation: 109 strains or lines available

References

• Original: J:40601 Williamson RA, et al., Dystroglycan is essential for early embryonic development: disruption of Reichert's membrane in Dag1-null mice. Hum Mol Genet. 1997 Jun;6(6):831-41
• All: 6 reference(s)