Fcer2a^tm1Putt
Targeted Allele Detail

Summary

• Symbol: Fcer2a^tm1Putt
• Name: Fc receptor, IgE, low affinity II, alpha polypeptide; targeted mutation 1, Herman van der Putten
• MGI ID: MGI:1934933
• Synonyms: CD23-
• Gene: Fcer2a Location: Chr8:3731737-3744175 bp, - strand Genetic Position: Chr8, 1.92 cM, cytoband A1
• Alliance: Fcer2a^tm1Putt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69547
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 800 bp of the gene including part of exon 10, all of exon 11, and part of exon 12. The deleted sequences encode the carboxy-terminus, including the lectin homology domain with sequences that bind the C epsilon domain of IgE. Northern blot analysis of spleen did not detect transcript in homozygous mutant mice. Flow cytometric analysis did not detect protein on the surface of splenic B cells in homozygous mutant mice. Immunocytochemical analysis did not detect protein in asteroid dentritic cells near the corticomedullary junction of homozygous mutant thymus. (J:69547)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fcer2a Mutation: 24 strains or lines available

References

• Original: J:69547 Stief A, et al., Mice deficient in CD23 reveal its modulatory role in IgE production but no role in T and B cell development. J Immunol. 1994 Apr 1;152(7):3378-90
• All: 2 reference(s)