Traf3^tm1Bal
Targeted Allele Detail

Summary

• Symbol: Traf3^tm1Bal
• Name: TNF receptor-associated factor 3; targeted mutation 1, David Baltimore
• MGI ID: MGI:2135257
• Synonyms: TRAF3^-
• Gene: Traf3 Location: Chr12:111132804-111233587 bp, + strand Genetic Position: Chr12, 60.94 cM, cytoband F
• Alliance: Traf3^tm1Bal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37065
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced the first two exons. Western blot analysis using a polyclonal antibody against the C-terminal portion did not detect protein in three tissues of homozygous mutant mice. Northern blot analysis using a cDNA fragment that encodes the C-terminus did not detect mRNA in brain of homozygous mutant mice. (J:37065)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Traf3^tm1Bal/Traf3^tm1Bal	involves: 129S4/SvJae * C57BL/6J
cx2	Nfkb2^tm2Brv/Nfkb2^tm2Brv Traf3^tm1Bal/Traf3^tm1Bal	involves: 129S1/Sv * 129S4/SvJae * C57BL/6
cx3	Nfkb2^tm2Brv/Nfkb2^+ Traf3^tm1Bal/Traf3^tm1Bal	involves: 129S1/Sv * 129S4/SvJae * C57BL/6

Phenotypes:

Affected Systems	hm1	cx2	cx3
show or hide all annotated terms
endocrine/exocrine glands	√
decreased thymocyte number	√
growth/size/body	√
decreased body size	√
decreased body weight	√
hematopoietic system	√
abnormal B cell differentiation	√
decreased leukocyte cell number	√
decreased thymocyte number	√
decreased double-positive T cell number	√
spleen hypoplasia	√
abnormal T cell physiology	√
homeostasis/metabolism	√
hypoglycemia	√
increased circulating corticosterone level	√
immune system	√	N
immune system phenotype		N
abnormal B cell differentiation	√
decreased leukocyte cell number	√
decreased thymocyte number	√
decreased double-positive T cell number	√
spleen hypoplasia	√
abnormal T cell physiology	√
mortality/aging	√	N	√
mortality/aging		N
postnatal lethality, complete penetrance	√		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Traf3 Mutation: 42 strains or lines available

References

• Original: J:37065 Xu Y, et al., Targeted disruption of TRAF3 leads to postnatal lethality and defective T-dependent immune responses. Immunity. 1996 Nov;5(5):407-15
• All: 9 reference(s)