Pdgfrb^tm2Sor
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^tm2Sor
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 2, Philippe Soriano
• MGI ID: MGI:2135519
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^tm2Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20016
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A promoterless beta-galactosidase/neomycin gene fusion (beta-geo) was inserted in frame into the exon coding for the signal peptide. Southern blot analysis demonstrated that three tandem copies of the beta-geo gene were present in a concatamer. Northern blot analysis failed to detect a PDGFbetaR transcript in homozygous mutants. Western blot analysis of total protein failed to detect any immunoreactive PDGFbetaR in homozygous mutants. (J:20016)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 87 strains or lines available

References

• Original: J:20016 Soriano P, Abnormal kidney development and hematological disorders in PDGF beta-receptor mutant mice. Genes Dev. 1994 Aug 15;8(16):1888-96
• All: 1 reference(s)