Pdgfrb^tm4Sor
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^tm4Sor
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 4, Philippe Soriano
• MGI ID: MGI:2135533
• Synonyms: F3
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^tm4Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66516
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A to T transversions in the sequences corresponding to codons 739 and 750 were introduced that altered the corresponding amino acids from tyrosine to phenylalanine (Y739F, Y750F). In addition, a mutation was introduced that altered the sequences of the codon corresponding to amino-acid 1020 from a tyrosine to an isoleucine (Y1020I) (DNA alteration not specified). These mutations are predicted to disrupt the PI3-K and PLC-gamma binding sites in the protein. A loxP flanked neomycin cassette was inserted into an adjacent intron. Removal of this neomycin cassette had no effect on the phenotype, and analysis of mice with or without the neomycin casstte was not distinguished in the paper. (J:66516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 87 strains or lines available

References

• Original: J:66516 Tallquist MD, et al., Retention of PDGFR-beta function in mice in the absence of phosphatidylinositol 3'-kinase and phospholipase cgamma signaling pathways. Genes Dev. 2000 Dec 15;14(24):3179-90
• All: 3 reference(s)