Pdgfrb^tm6Sor
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^tm6Sor
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 6, Philippe Soriano
• MGI ID: MGI:2135535
• Synonyms: betaKI
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^tm6Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67735
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Mutation details: A cDNA encoding PDGFbetaR was targeted into the Pdgfrb locus. This allele serves as a control for Pdgfrb^tm5Sor. The cDNA was inserted in frame into a Sma1 site in the second coding exon. Western blot analysis indicated that expression of the targeted Pdgfr locus was disrupted and the introduced receptor was expressed. (J:67735)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 87 strains or lines available

References

• Original: J:67735 Klinghoffer RA, et al., The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions. Mol Cell. 2001 Feb;7(2):343-54
• All: 1 reference(s)