Kras^tm2Tyj
Targeted Allele Detail

Summary

• Symbol: Kras^tm2Tyj
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 2, Tyler Jacks
• MGI ID: MGI:2135928
• Synonyms: K-ras2^LA1, K-ras^LA1, Kras^LA1
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm2Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68981
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Insertion of the targeting vector results in a duplication of exon 1 with a neomycin resistance gene cassette between the duplicate exons. The upstream (inserted) exon 1 contains an activating glycine to aspartic acid mutation at codon 12 (p.G12D). In the absence of intrachromosomal recombination, this allele does not produce any protein and mutant homozygotes die during embryogenesis. In heterozygotes, when intrachromosomal recombination occurs in vivo, half of the recombination events produce an active mutant allele coding for the p.G12D amino acid substitution and half produce a wild-type allele. Expression of G12D protein results in the formation of lung tumors and death at ~300 days (10 months). (J:68981)

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm2Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:68981 Johnson L, et al., Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature. 2001 Apr 26;410(6832):1111-6
• All: 45 reference(s)