Kras^tm3Tyj
Targeted Allele Detail

Summary

• Symbol: Kras^tm3Tyj
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 3, Tyler Jacks
• MGI ID: MGI:2136169
• Synonyms: Kras^G12D, K-ras^LA2, Kras^LA2, KrasLA2, Kras^LA2-G12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm3Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68981
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Insertion of the targeting vector results in a duplication of exon 1 with a neomycin resistance gene cassette between the duplicate exons. Both copies of exon 1 contain an activating glycine to aspartic acid mutation at codon 12 (p.G12D). In the absence of intrachromosomal recombination, this allele does not produce any protein and mutant homozygotes die during embryogenesis. In heterozygotes, when intrachromosomal recombination occurs in vivo, all of the recombination events produce an active p.G12D allele. Expression of G12D protein results in the formation of lung tumors and death at ~200 days (7 months). (J:68981)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm3Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:68981 Johnson L, et al., Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature. 2001 Apr 26;410(6832):1111-6
• All: 100 reference(s)