Klc1^tm1Gsn
Targeted Allele Detail

Summary

• Symbol: Klc1^tm1Gsn
• Name: kinesin light chain 1; targeted mutation 1, Lawrence S B Goldstein
• MGI ID: MGI:2136259
• Gene: Klc1 Location: Chr12:111725283-111774278 bp, + strand Genetic Position: Chr12, 61.13 cM
• Alliance: Klc1^tm1Gsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57877
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES Beta-geo cassette replaced the exon that encodes the entire first TPR domain and part of the second TPR domain. Western analysis of crude cytoplasmic brain extracts of homozygous mutant mice detected dramatically reduced levels of full length KLC1, as well as very small amounts of truncated KLC1. Immunoprecipitation studies did not detect either form of KLC1 in KIF5A or KIF5B coprecipitates of brain extracts of homozygous mutant mice. Immunofluorescence studies of sensory and motor neuron cell bodies in dorsal root ganglion and spinal cord of homozygous mutant mice detected very faint KCL1 staining. (J:57877)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Klc1 Mutation: 54 strains or lines available

References

• Original: J:57877 Rahman A, et al., Defective kinesin heavy chain behavior in mouse kinesin light chain mutants. J Cell Biol. 1999 Sep 20;146(6):1277-88
• All: 10 reference(s)