Irs1^tm1Jos
Targeted Allele Detail

Summary

• Symbol: Irs1^tm1Jos
• Name: insulin receptor substrate 1; targeted mutation 1, Joslin Diabetes Center
• MGI ID: MGI:2136455
• Synonyms: Irs1-, IRS-1-, IRS-1 KO
• Gene: Irs1 Location: Chr1:82210822-82269137 bp, - strand Genetic Position: Chr1, 42.0 cM, cytoband C5
• Alliance: Irs1^tm1Jos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21374
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced bases -77 to + 179 of the gene, deleting the start codon and producing a shift in the reading frame. Western blots of liver and muscle extracts showed no detectable protein. (J:21374, J:169458)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irs1 Mutation: 52 strains or lines available

References

• Original: J:21374 Araki E, et al., Alternative pathway of insulin signalling in mice with targeted disruption of the IRS-1 gene [see comments]. Nature. 1994 Nov 10;372(6502):186-90
• All: 44 reference(s)