Vhl^tm1Jae
Targeted Allele Detail

Summary

• Symbol: Vhl^tm1Jae
• Name: von Hippel-Lindau tumor suppressor; targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:2136645
• Synonyms: 2-lox allele, VHL², VHL^+f, VHL^f, Vhlh², Vhlh 2-lox, Vhlh^2lox, Vhlh^+f, Vhlh^fl
• Gene: Vhl Location: Chr6:113600955-113608595 bp, + strand Genetic Position: Chr6, 52.81 cM
• Alliance: Vhl^tm1Jae page

Vascular tumors in Vhl^tm1.1Jae/Vhl⁺ livers and vascular lesions in Vhl^tm1Jae/Vhl^tm1Jae Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67505
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A CMV-hyTK selection cassette flanked by two loxP sites was inserted approximately 2.6 kb upstream of exon 1. A third loxP site was inserted into intron 1. The hygromycin cassette was deleted in ES cells by transient transfection with a vector driving expression of Cre recombinase. The resulting ES cell derivatives with two loxP sites flanking exon 1 were selected and injected into blastocysts to generate the Vhl^tm1Jae allele. (J:67505)

Disease models

Expression

In Mice Carrying this Mutation:	103 assay results 6 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	22 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Vhl^tm1Jae

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Vhl Mutation: 17 strains or lines available

References

• Original: J:67505 Haase VH, et al., Vascular tumors in livers with targeted inactivation of the von Hippel-Lindau tumor suppressor. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1583-8
• All: 187 reference(s)