Ret^tm2Cos
Targeted Allele Detail

Summary

• Symbol: Ret^tm2Cos
• Name: ret proto-oncogene; targeted mutation 2, Frank Costantini
• MGI ID: MGI:2136895
• Synonyms: ret^MEN2B-neo
• Gene: Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
• Alliance: Ret^tm2Cos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60659
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: A T to C transition in codon 919, the equivalent codon to human 918, resulted in a protein which encoded threonine instead of methionine at this position (M919T). Silent mutations in codons 920 and 921 abolished a MunI endonuclease site. A loxP flanked neomycin cassette was inserted into an adjacent intron. (J:60659)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ret Mutation: 54 strains or lines available

References

• Original: J:60659 Smith-Hicks CL, et al., C-cell hyperplasia, pheochromocytoma and sympathoadrenal malformation in a mouse model of multiple endocrine neoplasia type 2B. EMBO J. 2000 Feb 15;19(4):612-22
• All: 1 reference(s)