En1^tm1Gld
Targeted Allele Detail

Summary

• Symbol: En1^tm1Gld
• Name: engrailed 1; targeted mutation 1, Martyn Goulding
• MGI ID: MGI:2137030
• Synonyms: En1-, En1^taulacZ, En1/tauLacZ, En1tau-LacZ, En1^tlZ
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^tm1Gld page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52637
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A tau-lacZ reporter gene cassette was inserted into the first exon, replacing sequences encoding the endogenous methionine initiation codon and the first 72 amino acids. Heterozygous mice expressed tau-beta-galactosidase that was indistinguishable from the endogenous expression pattern of En1. (J:52637)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:52637 Saueressig H, et al., Engrailed-1 and netrin-1 regulate axon pathfinding by association interneurons that project to motor neurons. Development. 1999 Oct;126(19):4201-12
• All: 10 reference(s)