Ephx1^tm1Gonz
Targeted Allele Detail

Summary

• Symbol: Ephx1^tm1Gonz
• Name: epoxide hydrolase 1, microsomal; targeted mutation 1, Frank J Gonzalez
• MGI ID: MGI:2137236
• Synonyms: mEH-
• Gene: Ephx1 Location: Chr1:180817121-180845134 bp, - strand Genetic Position: Chr1, 84.48 cM
• Alliance: Ephx1^tm1Gonz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56886
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 0.7 kb of sequence containing part of exon 2. Northern blot analysis detected a shorter mRNA transcript produced by this allele. However, western blot analysis of extracts of liver microsomes derived from homozygous mice did not detect any encoded protein. (J:56886)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ephx1 Mutation: 31 strains or lines available

Notes

The ES cell line, purchased from Genome Systems (St. Louis), was derived from 129X1/Sv. The name of the line was not specified.

References

• Original: J:56886 Miyata M, et al., Targeted disruption of the microsomal epoxide hydrolase gene. Microsomal epoxide hydrolase is required for the carcinogenic activity of 7,12-dimethylbenz[a]anthracene. J Biol Chem. 1999 Aug 20;274(34):23963-8
• All: 5 reference(s)