Nr1h4^tm1Gonz
Targeted Allele Detail

Summary

• Symbol: Nr1h4^tm1Gonz
• Name: nuclear receptor subfamily 1, group H, member 4; targeted mutation 1, Frank Gonzalez
• MGI ID: MGI:2137330
• Synonyms: FXR^-, FXRalpha^-, FXR/BAR -, Fxr^tm1Gonz
• Gene: Nr1h4 Location: Chr10:89290096-89369447 bp, - strand Genetic Position: Chr10, 44.98 cM
• Alliance: Nr1h4^tm1Gonz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64792
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Mice with a targeted deletion of the last exon encoding the ligand binding domain and all of the 3' untranslated region were produced as follows: A loxP site was inserted in the intron 5' to the last exon and a loxP-flanked neomycin cassette was inserted 3' to the last exon. After production of chimeric founder mice, F1 mice were mated to Tg(EIIa-Cre)1Lmgd mice to produce offspring that carried a recombined deletion of the last exon and neomycin cassette. No Nr1h4 protein product is detected in liver tissue from these null mice although an aberrant transcript appears to be generated. (J:64792)

Disease models

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Nr1h4^tm1Gonz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Nr1h4 Mutation: 43 strains or lines available

Notes

The ES cell line was not specified, but was purchased from Genome Systems (St. Louis, MO). (Note added 7/26/01: Genome Systems was bought by Incyte.)

References

• Original: J:64792 Sinal CJ, et al., Targeted disruption of the nuclear receptor FXR/BAR impairs bile acid and lipid homeostasis. Cell. 2000 Sep 15;102(6):731-44
• All: 186 reference(s)