Sparc^tm1Cam
Targeted Allele Detail

Summary

• Symbol: Sparc^tm1Cam
• Name: secreted acidic cysteine rich glycoprotein; targeted mutation 1, University of Cambridge
• MGI ID: MGI:2137432
• Gene: Sparc Location: Chr11:55284985-55310906 bp, - strand Genetic Position: Chr11, 33.04 cM, cytoband B1
• Alliance: Sparc^tm1Cam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47014
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Replacement of exon 6 with a splice acceptor-lacZ gene followed by a neomycin resistance cassette. Northern analysis did not detect transcript in E15.5 homozygous embryos. (J:47014)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sparc Mutation: 76 strains or lines available

References

• Original: J:47014 Gilmour DT, et al., Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens. EMBO J. 1998 Apr 1;17(7):1860-70
• All: 9 reference(s)