Gja8^tm1Scr
Targeted Allele Detail

Summary

• Symbol: Gja8^tm1Scr
• Name: gap junction protein, alpha 8; targeted mutation 1, The Scripps Institute
• MGI ID: MGI:2137525
• Synonyms: alpha8(-), Gja8^m1
• Gene: Gja8 Location: Chr3:96820882-96833336 bp, - strand
• Alliance: Gja8^tm1Scr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73651
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ gene, followed by a neomycin selection cassette, was inserted in-frame after the sixth codon. Western blot analysis demonstrated that no GJA8 protein is detectable in lens homogenates derived from homozygous mice. Beta-galactosidase is expressed from this allele under the control of the endogenous promoter. (J:73651)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja8 Mutation: 33 strains or lines available

References

• Original: J:73651 Rong P, et al., Disruption of Gja8 (alpha8 connexin) in mice leads to microphthalmia associated with retardation of lens growth and lens fiber maturation. Development. 2002 Jan;129(1):167-74
• All: 9 reference(s)