Apoe^tm1Bres
Targeted Allele Detail

Summary

• Symbol: Apoe^tm1Bres
• Name: apolipoprotein E; targeted mutation 1, Jan L Breslow
• MGI ID: MGI:2137814
• Synonyms: apo E-, apoE^-, EKO, epsilon-
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^tm1Bres page

Gross appearance of aortic arches of Apoe^tm1Bres/Apoe^tm1Bres, Plg^tm1Jld/Plg^tm1Jld and Apoe^tm1Bres/Apoe^tm1Bres Plg^tm1Jld/Plg^tm1Jld mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:2809
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 1.4kb of the gene, including part of the promoter through part of the second intron. Northern blot analysis using a mouse exon 4 riboprobe did not detect wild-type message in livers from homozygous mutant mice, but did detect a longer transcript that the authors propose is a neo-Apoe fusion product. Gradient SDS-PAGE analysis did not detect full size Apoe associated with any fractions from plasma lipoproteins from homozygous mutant mice. (J:2809)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 9 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 158 strains or lines available

Notes

In Apoe^tm1Bres mutant mice also carrying Csf1^op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:29024).

References

• Original: J:2809 Plump AS, et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell. 1992 Oct 16;71(2):343-53
• All: 135 reference(s)