Ptgir^tm1Sna
Targeted Allele Detail

Summary

• Symbol: Ptgir^tm1Sna
• Name: prostaglandin I receptor (IP); targeted mutation 1, Shuh Narumiya
• MGI ID: MGI:2137832
• Synonyms: IP-, IPKO
• Gene: Ptgir Location: Chr7:16640442-16644828 bp, + strand Genetic Position: Chr7, 9.15 cM
• Alliance: Ptgir^tm1Sna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:42275
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 2.4kb of sequence containing parts of the putative exons II and III, which encode the sixth transmembrane domain. A functional assay did not detect the protein in homozygous mutant mice. (J:42275)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ptgir^tm1Sna

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ptgir Mutation: 32 strains or lines available

Notes

Loss of Ptgir was shown functionally: e.g., a prostacyclin analogue (cicaprost) was used to inhibit platelet aggregation induced by ADP (platelet aggregation was inhibited in wild-type mice, but not in homozygous mutant mice), relaxation of smooth muscle in aortic rings of wild-type and homozygous mutant mice was measured after stimulation with a prostacyclin analogue (cicaprost, again, homozygous mutant mice showed no relaxation upon stimulation). Specificity of the knock-out was demonstrated by functional assay using other prostaglandins (e.g., PGE2). No differences between wild-type and homozygous mutant deficient mice were seen in response to PGE2.

References

• Original: J:42275 Murata T, et al., Altered pain perception and inflammatory response in mice lacking prostacyclin receptor. Nature. 1997 Aug 14;388(6643):678-82
• All: 48 reference(s)