Calca^tm1Eme
Targeted Allele Detail

Summary

• Symbol: Calca^tm1Eme
• Name: calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1, Ronald Emeson
• MGI ID: MGI:2148538
• Synonyms: alpha-CGRP^-, alphaCGRP^-, Calca^tm1Jrs, CGRP -
• Gene: Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
• Alliance: Calca^tm1Eme page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57708
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: A mutation was introduced to create a stop codon immediately upstream of the coding region of the CGRP alternative splice transcript in exon 5. The codons for a 4-amino-acid peptide (DLQA) of unknown function that is normally proteolytically spliced from the C-terminus of the alphaCGRP prohormone, was duplicated and inserted after the codons for a dibasic proteolytic cleavage site (normally used to cleave CGRP from the alphaCGRP prohormone) and before the stop codon in order to preserve the expression of this peptide. Thus, only the CGRP expression is knocked-out and the expression of the other neuropeptides created from this locus (calcitonin, etc.) is left undisturbed. (J:57708)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Calca Mutation: 23 strains or lines available

References

• Original: J:57708 Lu JT, et al., Mice lacking alpha-calcitonin gene-related peptide exhibit normal cardiovascular regulation and neuromuscular development. Mol Cell Neurosci. 1999 Aug;14(2):99-120
• All: 17 reference(s)