Utrn^tm1Jrs
Targeted Allele Detail

Summary

• Symbol: Utrn^tm1Jrs
• Name: utrophin; targeted mutation 1, Joshua R Sanes
• MGI ID: MGI:2148539
• Synonyms: uko, utrn-
• Gene: Utrn Location: Chr10:12257932-12745109 bp, - strand Genetic Position: Chr10, 3.77 cM
• Alliance: Utrn^tm1Jrs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39218
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 2.7 kb of sequence including 52 bp of the exon that encodes the beginning of the C-terminal cysteine rich region. RT-PCR analysis demonstrated that a mutant transcript carrying the deletion was made in homozygous and heterozygous mice. Western blot analysis on extracts of muscle or lung tissue derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:39218)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Utrn Mutation: 304 strains or lines available

References

• Original: J:39218 Grady RM, et al., Subtle neuromuscular defects in utrophin-deficient mice. J Cell Biol. 1997 Feb 24;136(4):871-82
• All: 59 reference(s)