Ctnnb1^tm2Wbm
Targeted Allele Detail

Summary

• Symbol: Ctnnb1^tm2Wbm
• Name: catenin beta 1; targeted mutation 2, Walter Birchmeier
• MGI ID: MGI:2148591
• Synonyms: beta-cat^lacZ(neo)
• Gene: Ctnnb1 Location: Chr9:120762466-120789573 bp, + strand Genetic Position: Chr9, 72.19 cM
• Alliance: Ctnnb1^tm2Wbm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60391
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ gene was inserted in-frame into the gene, fused to the endogenous transcription start site. A neomycin selection cassette followed the lacZ gene. Northern blot analysis on RNA derived from homozygous ES cells demonstrated that no detectable transcript was produced from this allele, and western blot analysis on homozygous ES cells confirmed that no stable protein is produced from this allele (data not shown). (J:60391)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctnnb1 Mutation: 49 strains or lines available

References

• Original: J:60391 Huelsken J, et al., Requirement for beta-catenin in anterior-posterior axis formation in mice. J Cell Biol. 2000 Feb 7;148(3):567-78
• All: 3 reference(s)