Ctnnb1^tm4Wbm
Targeted Allele Detail

Summary

• Symbol: Ctnnb1^tm4Wbm
• Name: catenin beta 1; targeted mutation 4, Walter Birchmeier
• MGI ID: MGI:2148594
• Synonyms: beta-catenin^flox, beta-catenin^lox, beta-cat lox, beta-cat^loxEx3-6, Ctnnb1^fl
• Gene: Ctnnb1 Location: Chr9:120762466-120789573 bp, + strand Genetic Position: Chr9, 72.19 cM
• Alliance: Ctnnb1^tm4Wbm page

Ctnnb1^tm4Wbm/Ctnnb1^tm4Wbm Tg(Pdx1-cre)6Tuv/? pancreata display extensive loss of exocrine tissue

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69463
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into the second intron and a loxP-flanked neomycin selection cassette was inserted into the sixth intron. The neomycin cassette was removed by transient expression of Cre recombinase in ES cells prior to the production of chimeric mice, leaving two loxP sites that flank exons 3-6 in the final allele. Northern blot analysis demonstrated that a transcript of wild-type length is produced from this allele, and western blot analysis confirmed that a normal protein is made. (J:69463)

Expression

In Mice Carrying this Mutation:	35 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ctnnb1 Mutation: 49 strains or lines available

References

• Original: J:69463 Huelsken J, et al., beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell. 2001 May 18;105(4):533-45
• All: 81 reference(s)