Fabp4^tm1Brsp
Targeted Allele Detail

Summary

• Symbol: Fabp4^tm1Brsp
• Name: fatty acid binding protein 4, adipocyte; targeted mutation 1, Bruce M Spiegelman
• MGI ID: MGI:2148887
• Synonyms: A-FABP/aP2, aP2-
• Gene: Fabp4 Location: Chr3:10269406-10273653 bp, - strand Genetic Position: Chr3, 2.56 cM
• Alliance: Fabp4^tm1Brsp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70953
• Parent Cell Line: CC1.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a neomycin cassette disrupted the gene after the transcription start site and before a translation start site at a Pst I site. Absence of mRNA expression in adipose tissue was shown by Northern blot and RT-PCR in homozygous mice. Immunoblot analysis of adipose tissue showed no detectable protein. (J:70953)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fabp4 Mutation: 44 strains or lines available

References

• Original: J:70953 Johnson RS, et al., Targeting of nonexpressed genes in embryonic stem cells via homologous recombination. Science. 1989 Sep 15;245(4923):1234-6
• All: 52 reference(s)