Itgb5^tm1Des
Targeted Allele Detail

Summary

• Symbol: Itgb5^tm1Des
• Name: integrin beta 5; targeted mutation 1, Dean Sheppard
• MGI ID: MGI:2149063
• Synonyms: beta5-
• Gene: Itgb5 Location: Chr16:33650035-33769708 bp, + strand Genetic Position: Chr16, 24.22 cM
• Alliance: Itgb5^tm1Des page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59991
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced part of an exon of the gene that corresponds to amino acids 114 to 230 in the reported human sequence, and an adjacent intron. Northern analysis using a murine cDNA probe did not detect transcript in cultured keratinocytes from homozygous mutant mice. Immunoprecipitation studies using antiserum raised against the cytoplasmic domain of human did not detect protein in lysates of cultured keratinocytes from homozygous mutant mice. (J:59991)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itgb5 Mutation: 51 strains or lines available

References

• Original: J:59991 Huang X, et al., Normal development, wound healing, and adenovirus susceptibility in beta5-deficient mice. Mol Cell Biol. 2000 Feb;20(3):755-9
• All: 26 reference(s)