Add2^tm1Feb
Targeted Allele Detail

Summary

• Symbol: Add2^tm1Feb
• Name: adducin 2; targeted mutation 1, Francisco E Baralle
• MGI ID: MGI:2149087
• Gene: Add2 Location: Chr6:86005663-86101391 bp, + strand Genetic Position: Chr6, 37.55 cM
• Alliance: Add2^tm1Feb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63047
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exons 9 to 13. Northern blot analysis using a full-length rat cDNA probe detected transcripts smaller than wild-type in brain and spleen of homozygous animals. Western blot analysis did not detect wild-type or deleted forms of protein in brain, spleen, or erythrocyte ghosts from homozygous mice. (J:63047)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Add2 Mutation: 43 strains or lines available

References

• Original: J:63047 Muro AF, et al., Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice. Blood. 2000 Jun 15;95(12):3978-85
• All: 5 reference(s)