Epha4^tm1Pch
Targeted Allele Detail

Summary

• Symbol: Epha4^tm1Pch
• Name: Eph receptor A4; targeted mutation 1, Patrick Charnay
• MGI ID: MGI:2149729
• Synonyms: EphA4-, EphA4^KO, Epha4^lacZ
• Gene: Epha4 Location: Chr1:77343822-77491725 bp, - strand Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
• Alliance: Epha4^tm1Pch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62985
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, which encodes the first 30 amino acids, and part of the first intron were replaced by a lacZ-PGK-neomycin resistance cassette. The lacZ-coding sequence was placed immediately downstream of and in frame with the initiating codon. In addition, a frameshift mutation was introduced into exon 3. Western blot analysis did not detect protein in heads of homozygous mutant E14.5 embryos. Immunohistochemistry did not detect protein in the brachial region of homozygous mutant E11.5 embryos. (J:62985)

Expression

In Mice Carrying this Mutation:

37 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epha4 Mutation: 67 strains or lines available

References

• Original: J:62985 Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
• All: 21 reference(s)