Ephb2^tm1Paw
Targeted Allele Detail

Summary

• Symbol: Ephb2^tm1Paw
• Name: Eph receptor B2; targeted mutation 1, Tony Pawson
• MGI ID: MGI:2149765
• Synonyms: EphB2-, EphB2^delta, Ephb2^KO, Nuk¹
• Gene: Ephb2 Location: Chr4:136374850-136563299 bp, - strand Genetic Position: Chr4, 69.0 cM, cytoband D-E
• Alliance: Ephb2^tm1Paw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34200
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced 1.4kb of sequence including the exon that encodes amino acids 29-50, and introduced a frame shift downstream of the insertion. In an immune-complex in vitro tyrosine kinase assay, antiserum raised against the C-terminal of the protein did not detect wild-type protein in lysates of homozygous mutant E10.5 embryos. (J:34200)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ephb2 Mutation: 68 strains or lines available

References

• Original: J:34200 Henkemeyer M, et al., Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell. 1996 Jul 12;86(1):35-46
• All: 60 reference(s)