Aqp5^tm1Ask
Targeted Allele Detail

Summary

• Symbol: Aqp5^tm1Ask
• Name: aquaporin 5; targeted mutation 1, Alan S Verkman
• MGI ID: MGI:2150059
• Synonyms: AQP5-
• Gene: Aqp5 Location: Chr15:99488909-99492710 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Aqp5^tm1Ask page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56302
• Parent Cell Line: CB1-4 (ES Cell)
• Strain of Origin: C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.8kb neomycin resistance cassette replaced parts of exon 2 and 4, all of exon 3, and all of introns 2 and 3. Northern blots of submandibular gland homogenates from homozygous mutant mice showed no detectable full-length Aqp5 transcripts. Immunocytochemistry of the salivary glands from homozygous mutant mice showed no detectable Aqp5 protein. (J:56302)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp5 Mutation: 19 strains or lines available

References

• Original: J:56302 Ma T, et al., Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels. J Biol Chem. 1999 Jul 16;274(29):20071-4
• All: 19 reference(s)