Nos3^tm1Plh
Targeted Allele Detail

Summary

• Symbol: Nos3^tm1Plh
• Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, Paul L Huang
• MGI ID: MGI:2150145
• Synonyms: eNOS^-, eNOS KO, NOSIII^-
• Gene: Nos3 Location: Chr5:24569808-24589472 bp, + strand Genetic Position: Chr5, 11.32 cM
• Alliance: Nos3^tm1Plh page

Renal tubular apoptosis, regeneration, glomerulosclerosis and glomerular thrombus formation in Nos1^tm1Plh/Nos1^tm1Plh Nos2^tm1Mrl/Nos2^tm1Mrl Nos3^tm1Plh/Nos3^tm1Plh mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:28778
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced 3 exons that encode the NADPH ribose and adenine binding sites of the protein (amino acids 1010 - 1144). Western blots of protein extracts of brain, heart, lung, and aorta/vena cava from homozygous mutant mice showed no detectable encoded protein. (J:28778)
• Inheritance: Dominant

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	28 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nos3 Mutation: 58 strains or lines available

References

• Original: J:28778 Huang PL, et al., Hypertension in mice lacking the gene for endothelial nitric oxide synthase [see comments]. Nature. 1995 Sep 21;377(6546):239-42
• All: 128 reference(s)